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Notwithstanding The variability of techniques which were launched to beat artefacts connected with facts compositionality, they've not been adopted to the human microbiome investigate. Of Be aware, Not one of the clinical trials revealed throughout the past calendar year (apart from those particularly addressing the compositionality trouble [five, 6]) used quantitative microbiome profiling. Here, we current how quantitative PCR (qPCR)-dependent bacterial enumeration is usually integrated to NGS pipelines to deliver a feasible method of estimate complete abundances from NGS facts, and for this reason encourage the use of quantitative microbiome profiling in the field of human microbiome.
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